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human refseq build 45 protein sequence database  (Thermo Fisher)


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    Structured Review

    Thermo Fisher human refseq build 45 protein sequence database
    Human Refseq Build 45 Protein Sequence Database, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/refseq+protein+sequence+database/pm25756516-113-6-30
    Average 90 stars, based on 1 article reviews
    human refseq build 45 protein sequence database - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Calcium calmodulin dependent kinase kinase 2 - a novel therapeutic target for gastric adenocarcinoma.
    Article Snippet: The data were then searched against human RefSeq Build 45 protein sequence database (containing 34,349 sequences with common contaminants) using Mascot (version 2.2) and Sequest search algorithms through Proteome Discoverer (Thermo Scientific, Bremen, Germany, version 1.3.0.339). .. The data were then searched against human RefSeq Build 45 protein sequence database (containing 34,349 sequences with common contaminants) using Mascot (version 2.2) and Sequest search algorithms through Proteome Discoverer (Thermo Scientific, Bremen, Germany, version 1.3.0.339). ..

    Plasmid Preparation:

    Article Title: Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins
    Article Snippet: .. A pENTR plasmid expressing signal peptide–SNAP-Gpr161 (with Gpr161 lacking the first 27 aa from NCBI RefSeq database accession no. NP_001074595 ) was synthesized by Thermo Fisher Scientific, and C-terminal expression constructs were generated by Gateway cloning into pG-LAP5. .. RPE hTERT and ARPE-19 cells were grown in DMEM F12 media with 10% FBS (Sigma-Aldrich), 0.05 mg/ml penicillin, 0.05 mg/ml streptomycin, and 4.5 mM glutamine. mIMCD-K2 cells were a gift from B. Stanton (Dartmouth College, Hanover, NH).

    Expressing:

    Article Title: Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins
    Article Snippet: .. A pENTR plasmid expressing signal peptide–SNAP-Gpr161 (with Gpr161 lacking the first 27 aa from NCBI RefSeq database accession no. NP_001074595 ) was synthesized by Thermo Fisher Scientific, and C-terminal expression constructs were generated by Gateway cloning into pG-LAP5. .. RPE hTERT and ARPE-19 cells were grown in DMEM F12 media with 10% FBS (Sigma-Aldrich), 0.05 mg/ml penicillin, 0.05 mg/ml streptomycin, and 4.5 mM glutamine. mIMCD-K2 cells were a gift from B. Stanton (Dartmouth College, Hanover, NH).

    Synthesized:

    Article Title: Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins
    Article Snippet: .. A pENTR plasmid expressing signal peptide–SNAP-Gpr161 (with Gpr161 lacking the first 27 aa from NCBI RefSeq database accession no. NP_001074595 ) was synthesized by Thermo Fisher Scientific, and C-terminal expression constructs were generated by Gateway cloning into pG-LAP5. .. RPE hTERT and ARPE-19 cells were grown in DMEM F12 media with 10% FBS (Sigma-Aldrich), 0.05 mg/ml penicillin, 0.05 mg/ml streptomycin, and 4.5 mM glutamine. mIMCD-K2 cells were a gift from B. Stanton (Dartmouth College, Hanover, NH).

    Construct:

    Article Title: Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins
    Article Snippet: .. A pENTR plasmid expressing signal peptide–SNAP-Gpr161 (with Gpr161 lacking the first 27 aa from NCBI RefSeq database accession no. NP_001074595 ) was synthesized by Thermo Fisher Scientific, and C-terminal expression constructs were generated by Gateway cloning into pG-LAP5. .. RPE hTERT and ARPE-19 cells were grown in DMEM F12 media with 10% FBS (Sigma-Aldrich), 0.05 mg/ml penicillin, 0.05 mg/ml streptomycin, and 4.5 mM glutamine. mIMCD-K2 cells were a gift from B. Stanton (Dartmouth College, Hanover, NH).

    Generated:

    Article Title: Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins
    Article Snippet: .. A pENTR plasmid expressing signal peptide–SNAP-Gpr161 (with Gpr161 lacking the first 27 aa from NCBI RefSeq database accession no. NP_001074595 ) was synthesized by Thermo Fisher Scientific, and C-terminal expression constructs were generated by Gateway cloning into pG-LAP5. .. RPE hTERT and ARPE-19 cells were grown in DMEM F12 media with 10% FBS (Sigma-Aldrich), 0.05 mg/ml penicillin, 0.05 mg/ml streptomycin, and 4.5 mM glutamine. mIMCD-K2 cells were a gift from B. Stanton (Dartmouth College, Hanover, NH).

    Cloning:

    Article Title: Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins
    Article Snippet: .. A pENTR plasmid expressing signal peptide–SNAP-Gpr161 (with Gpr161 lacking the first 27 aa from NCBI RefSeq database accession no. NP_001074595 ) was synthesized by Thermo Fisher Scientific, and C-terminal expression constructs were generated by Gateway cloning into pG-LAP5. .. RPE hTERT and ARPE-19 cells were grown in DMEM F12 media with 10% FBS (Sigma-Aldrich), 0.05 mg/ml penicillin, 0.05 mg/ml streptomycin, and 4.5 mM glutamine. mIMCD-K2 cells were a gift from B. Stanton (Dartmouth College, Hanover, NH).

    Tandem Mass Spectroscopy:

    Article Title: Discovery of noncanonical translation initiation sites through mass spectrometric analysis of protein N termini
    Article Snippet: .. MS/MS data obtained from LC-MS analyses were searched against the RefSeq human protein database (version 70) for ORF regions or customized databases for 5′ UTRs with 248 common contaminant proteins using SEQUEST search algorithms through Proteome Discoverer 2.1 platform (Thermo Scientific). ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Discovery of noncanonical translation initiation sites through mass spectrometric analysis of protein N termini
    Article Snippet: .. MS/MS data obtained from LC-MS analyses were searched against the RefSeq human protein database (version 70) for ORF regions or customized databases for 5′ UTRs with 248 common contaminant proteins using SEQUEST search algorithms through Proteome Discoverer 2.1 platform (Thermo Scientific). ..

    Mass Spectrometry:

    Article Title: Identification of miR-145 targets through an integrated omics analysis
    Article Snippet: .. Mass spectrometry raw data were searched against Human RefSeq protein database (Release 46) using Proteome Discoverer platform (Thermo, Bremen, Germany). ..

    Article Title: Identification of miR-145 targets through an integrated omics analysis
    Article Snippet: .. Mass spectrometry raw data were searched against Human RefSeq protein database (Release 46) using Proteome Discoverer platform (Thermo, Bremen, Germany). ..

    Polymerase Chain Reaction:

    Article Title: Rapid development of atherosclerosis in the world's smallest Microminipig fed a high-fat/high-cholesterol diet.
    Article Snippet: .. Genes investigated and primers used for PCR Gene Assay ID Custom made primers RefSeq GenBank LDLr SR-BI HMGCR SREBP-2 APOBEC-1 NPC1L1 GAPDH Ss03374441_u1 Ss03391104_m1 Ss03390147_m1 Ss03376492_u1 Ss03375435_u1 Forward: CCATGGTGTGACCATCCAGAT Reverse: AGTTGACAAAATTCCTCCAGCAGTA Probe: TGGGAGCCCCAGAGTA Forward: CCTGTTCGGAGCGAGTCTCTA Reverse: GAAAGAGGAAATAGTCGAGCAGGTA Probe: CTGCCCAAGGACTC NM_001206354.1 NM_213967.1 NM_001122988.1 XM_003126519.1 XM_003134893.1 NM_001206359.1 AF065990.1 AF467889.2 DQ432054.1 AY493571.1 AY307771.1 The assay IDs are listed for the predesigned gene expression assay (ABI). ..

    Gene Assay:

    Article Title: Rapid development of atherosclerosis in the world's smallest Microminipig fed a high-fat/high-cholesterol diet.
    Article Snippet: .. Genes investigated and primers used for PCR Gene Assay ID Custom made primers RefSeq GenBank LDLr SR-BI HMGCR SREBP-2 APOBEC-1 NPC1L1 GAPDH Ss03374441_u1 Ss03391104_m1 Ss03390147_m1 Ss03376492_u1 Ss03375435_u1 Forward: CCATGGTGTGACCATCCAGAT Reverse: AGTTGACAAAATTCCTCCAGCAGTA Probe: TGGGAGCCCCAGAGTA Forward: CCTGTTCGGAGCGAGTCTCTA Reverse: GAAAGAGGAAATAGTCGAGCAGGTA Probe: CTGCCCAAGGACTC NM_001206354.1 NM_213967.1 NM_001122988.1 XM_003126519.1 XM_003134893.1 NM_001206359.1 AF065990.1 AF467889.2 DQ432054.1 AY493571.1 AY307771.1 The assay IDs are listed for the predesigned gene expression assay (ABI). ..

    Gene Expression:

    Article Title: Rapid development of atherosclerosis in the world's smallest Microminipig fed a high-fat/high-cholesterol diet.
    Article Snippet: .. Genes investigated and primers used for PCR Gene Assay ID Custom made primers RefSeq GenBank LDLr SR-BI HMGCR SREBP-2 APOBEC-1 NPC1L1 GAPDH Ss03374441_u1 Ss03391104_m1 Ss03390147_m1 Ss03376492_u1 Ss03375435_u1 Forward: CCATGGTGTGACCATCCAGAT Reverse: AGTTGACAAAATTCCTCCAGCAGTA Probe: TGGGAGCCCCAGAGTA Forward: CCTGTTCGGAGCGAGTCTCTA Reverse: GAAAGAGGAAATAGTCGAGCAGGTA Probe: CTGCCCAAGGACTC NM_001206354.1 NM_213967.1 NM_001122988.1 XM_003126519.1 XM_003134893.1 NM_001206359.1 AF065990.1 AF467889.2 DQ432054.1 AY493571.1 AY307771.1 The assay IDs are listed for the predesigned gene expression assay (ABI). ..



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